Catalysis of lyase-isomerase PecE/PecF for several apophycobiliproteins / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 703-708, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-256135
ABSTRACT
Phycoerythrocyanin(PEC) lyase-isomerase PecE/PecF from Mastigocladus laminosus is the specific enzyme for biosynthesis of PEC alpha-subunit(alpha-PEC). In this work, the specificity of PecE/PecF on substrate apoproteins was reported. PecE/PecF could catalyse the reconstitution of phycocyanobilin(PCB) with apoproteins of alpha-PEC from two different subspecies of Mastigocladus laminosus, as well the site-directed mutated apoprotein of alpha-PEC with Trp at 128 to Phe in vitro, but could not catalyse the reconstitution of PCB with apoprotein of phycocyanin alpha-subunit(alpha-CPC) from Mastigocladus laminosus. The surfactant Triton X-100 had no effect for the reconstitution of alpha-PEC, while it could improve the reconstitution of PCB with apoprotein of alpha-CPC.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Apoproteínas
/
Farmacologia
/
Especificidade por Substrato
/
Proteínas de Bactérias
/
Proteínas
/
Catálise
/
Cianobactérias
/
Octoxinol
/
Complexos de Proteínas Captadores de Luz
/
Liases
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2002
Tipo de documento:
Artigo
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