The effect of antioxidants on the in vitro life-span of keratinocyte / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 630-633, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-256149
ABSTRACT
The effect of antioxidants on the in vitro life span of mouse keratinocytes was investigated in this work. It was found that the life span of the keratinocytes cultured in the medium supplemented with antioxidants was extended significantly. The most beneficial antioxidant used in this work was the mercaptoethanol, followed by the catalase and SOD. However, the growth rates of keratinocytes in vitro under all the experimental conditions still declined with the culture time. It was also found that the antioxidants added in the medium were also helpful to enhance the keratinocyte colony formation. In addition, the aging kinetics of the mouse epidermal keratinocytes in vitro were analyzed, and finally the aging rate constants corresponding to antioxidants used were calculated.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Superóxido Dismutase
/
Catalase
/
Queratinócitos
/
Divisão Celular
/
Células Cultivadas
/
Senescência Celular
/
Biologia Celular
/
Mercaptoetanol
/
Antioxidantes
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2002
Tipo de documento:
Artigo
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