Cloning of staphylococcal enterotoxin B gene and its highly expression in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 597-600, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-256157
ABSTRACT
An about 700 bp DNA fragment was amplified from genome DNA of S. aureus TSTw by PCR. This fragment was cloned into pGEM-7Zf(+) and the recombinant plasmid was transformed into E. coli DH5 alpha. The sequencing result of the recombinant plasmid demonstrated that it contains seb gene with 717 bp (without signal encoding region of 81 bp) which has the same nucleotide sequence as described in literature. The seb gene was cloned into expression vector 7ZTS and was transformed into E. coli JM109 (DE3). The expression level of SEB was as high as 33.3% of the cell total proteins.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes
/
Engenharia Genética
/
Clonagem Molecular
/
Enterotoxinas
/
Escherichia coli
/
Genética
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2002
Tipo de documento:
Artigo
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