Cloning and sequencing of lactase gene from Aspergillus candidus / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 566-571, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-256163
ABSTRACT
Genomic DNA and cDNA sequences of lactase from Aspergillus candidus were cloned. Sequences analysis revealed that the genomic DNA was 3458 bp containing eight introns, cDNA was 3015 bp and encoding a polypeptide of 1005 amino acid residues. Signal peptide was 19 amino acid residues, eleven potential N-glycosylation sites were assumed. Comparing the gene cDNA and amino acid sequences with other lactase sequences from various sources, it showed a very low homology with most of other sequences. Although the gene had a higher homology to Aspergillus oryzae lactase sequence, characterization of both enzymes exhibited obvious difference. The gene from Aspergillus candidus was a promising new gene for food industry.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Aspergillus
/
Dados de Sequência Molecular
/
Química
/
Sequência de Aminoácidos
/
Clonagem Molecular
/
Beta-Galactosidase
/
Homologia de Sequência
/
Lactase
/
Genética
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2002
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS