The changes of rare codons increase expression level of SEA / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 477-480, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-256181
ABSTRACT
The rare codons of a fragment in staphylococcal enterotoxin A gene were turned into the most high usage frequency codons in E. coli by overlap PCR technique. Genes of sea and seam were cloned into 7ZTS expression vector and transformed into JM109(DE3), respectively. The result shows that expression level of sea gene was very low, but the expression level of seam was as high as 15% of total cell proteins. The expression product shows activity of antitumor in vivo.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Proteínas Recombinantes
/
Códon
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Regulação Bacteriana da Expressão Gênica
/
Mutação Puntual
/
Tratamento Farmacológico
/
Eletroforese em Gel de Poliacrilamida
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2002
Tipo de documento:
Artigo
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