Expression of polyprotein of infectious bursal disease virus in Bombyx mori / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 472-476, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-256182
ABSTRACT
Segment A of the genome of infectious bursal disease virus(IBDV) encodes structure protein VP2 and VP3 and protease VP4. In this study a polyprotein gene of IBDV was inserted into a Bombyx mori baculovirus transfer vector pAcHLT--C and contransfected into BmN cells with linear genome DNA of virus Bm-BacPAK6. Dot hybridization suggested that the segment A of the virus genome was inserted in the genome of Bm-BacPAK6. The silkworm of fifth instars were infected by the recombinant virus and the immunogenicity of the infected larvae's blood were examined with ELISA, SDS-PAGE and Western blotting. It appears that the recombinant polyprotein has the property of immunoreactivity and the expression in larvae reached the pick 5-day post infection.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Bombyx
/
Proteínas Virais
/
Virologia
/
Ensaio de Imunoadsorção Enzimática
/
Expressão Gênica
/
Western Blotting
/
Vírus da Doença Infecciosa da Bursa
/
Poliproteínas
/
Eletroforese em Gel de Poliacrilamida
/
Vetores Genéticos
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2002
Tipo de documento:
Artigo
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