The construction of shuttle vectors of Brevibacillus brevis-Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 438-441, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-256189
ABSTRACT
The 5' region of the cell wall protein(CWP) gene containing multiple tandem promoters and the signal peptide-coding sequence was isolated by PCR from Br. brevis 50, and used to construct the shuttle vector pBKE50, which included the replication origin of pUB110 and the erythromycin-resistance gene of pGK12. The alpha-amylase gene of Bacillus subtilis 168 was ligated to pBKE50, producing plasmid pBKE50/alpha-amy. After the resulting plasmid was introduced into Br. brevis 50, soluble and biologically active alpha-amylase was secreted directly into the culture medium. The expression level of alpha-amylase in the recombinant Br. brevis 50 was twice higher than that of the donor strain.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Plasmídeos
/
Bacillus
/
Proteínas Recombinantes de Fusão
/
Resistência Microbiana a Medicamentos
/
Eritromicina
/
Clonagem Molecular
/
Escherichia coli
/
Alfa-Amilases
/
Vetores Genéticos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2002
Tipo de documento:
Artigo
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