Cloning and expression of the cecropin B-thanatin hybrid antimicrobial peptide in Escherichia coli / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 352-355, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-256204
ABSTRACT
A 44-residue hybrid peptide (CB (1-24)-Arg-Ser-Tyr-Tan (4-21)) incorporating 1-24 residues of cecropin B (CB) and 4-21 residues of thanatin (Tan) was designed and constructed. The CB-Tan gene was cloned into expression plasmid pGEX-3X and expressed in E. coli BL21. The fusion protein was purified by affinity chromatography. After digested with enterokinase the gene product released with antibacterial activity and gave one band in Tricine-SDS-PAGE.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Peptídeos Cíclicos
/
Farmacologia
/
Proteínas Recombinantes de Fusão
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Sequência de Aminoácidos
/
Clonagem Molecular
/
Proteínas de Insetos
/
Peptídeos Catiônicos Antimicrobianos
/
Escherichia coli
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2002
Tipo de documento:
Artigo
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