Morphology, immunohistochemistry and hTERC gene in-situ hybridization in Barrett's esophagus / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 4-9, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-256268
ABSTRACT
<p><b>OBJECTIVE</b>To study the clinicopathologic features and differential diagnosis of proximal gastric mucosa and mucosa of Barrett's esophagus (BE) in biopsy specimens.</p><p><b>METHOD</b>Thirty-eight cases of Barrett's esophagus (diagnosed using WHO criteria) and 44 cases of proximal gastric mucosa were studied by immunohistochemistry (for CK7, CK20, CK4, CK8, S-100 protein, MUC6, COX2 and bcl-2) and fluorescence in-situ hybridization (FISH) (for hTERC gene). The pathologic features were analyzed.</p><p><b>RESULTS</b>The differences in expression of CK7, CK20, MUC6, COX2 and bcl-2 between BE and proximal gastric mucosa with intestinal metaplasia were not statistically significant (P > 0.05). There was however a statistically significant difference in expression of S-100 protein (P < 0.05). The expression of CK7/CK4 and CK7/CK8 in BE showed positive correlation (P < 0.05). However, such correlation was not demonstrated in proximal gastric mucosa (P > 0.05). The results of hTERC gene expression by FISH showed a statistically significant difference between the two groups 57.9% (22/38) in BE and 13.6% (6/44) in proximal gastric mucosa (P < 0.05).</p><p><b>CONCLUSIONS</b>The significance of CK7 and CK20 expression is uncertain in the differential diagnosis between BE and proximal gastric mucosa. On the other hand, positivity for CK7/CK4/CK8 may support the diagnosis of BE and play a role in distinguishing between the two groups. S-100 protein expression and detection of hTERC gene amplification also contribute to the diagnosis of BE.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Esôfago de Barrett
/
RNA
/
Imuno-Histoquímica
/
Proteínas S100
/
Amplificação de Genes
/
Estudos Retrospectivos
/
Hibridização in Situ Fluorescente
/
Telomerase
/
Queratina-20
Tipo de estudo:
Estudo observacional
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2013
Tipo de documento:
Artigo
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