Effects of ezrin silencing on pancreatic cancer cell line Panc-1 / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 833-836, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-256281
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of ezrin silencing on pancreatic cancer cell line Panc-1.</p><p><b>METHODS</b>Pancreatic cancer cell line Panc-1 was transfected with ezrin silencing plasmid. The proliferation and the cell cycle status were determined by CCK-8 assay and flow cytometry analysis, respectively. Cellular membrane protrusions/microvilli formation were visualized by scanning election microscopy. Colony formation assay was used to determine the cell anchor-independent growth ability in vitro. Trans-filter migration and invasion assays were performed with 8 µm pore inserts in a 24-well BioCoat chamber with/without Matrigel.</p><p><b>RESULTS</b>Ezrin silencing decreased cellular protrusions/microvilli formation, anchorage-independent growth, cell migration and invasion, but had no effects on cell proliferation in vitro and cell cycle, in pancreatic cancer cell line Panc-1.</p><p><b>CONCLUSION</b>Ezrin expression affects the cellular protrusions/microvilli formation, anchorage-independent growth, cell migration and invasion in pancreatic cancer cell line Panc-1.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias Pancreáticas
/
Patologia
/
Plasmídeos
/
Transfecção
/
Movimento Celular
/
Extensões da Superfície Celular
/
Proteínas do Citoesqueleto
/
RNA Interferente Pequeno
/
Interferência de RNA
/
Linhagem Celular Tumoral
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2012
Tipo de documento:
Artigo
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