DNA microarray analysis of protective mechanism of buyang huanwu decoction on focal cerebral ischemia/reperfusion in rats / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 559-563, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-256307
ABSTRACT
<p><b>OBJECTIVE</b>To analyse the effects of buyang huanwu decoction (BYHWT) on differentially expressed genes during cerebral ischemia/reperfusion in rats with DNA microarray.</p><p><b>METHOD</b>cDNA microarray chips containing 512 cDNAs were made by Biostar Genechip Inc. Sprague-Dawley rats were subjected to 2 h of middle cerebral artery occlusion (MCAO) with an filament. Saline or BYHWT was given p.o. after onset of cerebral ischemia and brains were removed after 24 h of recirculation for mRNAs isolation. A differential measurment of mRNAs from post-ischemic and BYHWT treated animals was performed with microarray.</p><p><b>RESULT</b>Up-and down-regulated genes were 69 and 80 in ischemic group. Up-and down-regulated genes were 25 and 6 in BYHWT treated group.</p><p><b>CONCLUSION</b>BYHWT regulates the differential expression genes after focal brain ischemia/reperfusion in rats, due to its mechanism of protecting cerebral ischemia/reperfusion injury.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Medicamentos de Ervas Chinesas
/
Traumatismo por Reperfusão
/
Isquemia Encefálica
/
Ratos Sprague-Dawley
/
Fármacos Neuroprotetores
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Perfilação da Expressão Gênica
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2004
Tipo de documento:
Artigo
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