The effect of lishi No.5 formula on the growth of human neuroblastoma cell line SY5Y / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect of Lishi No.5 formula on the growth of human neuroblastoma cell line SY5Y and find out the most effective drug dose.</p><p><b>METHOD</b>SY5Y cells were administrated by Lishi No.5 formula in different concentration. MTT metabolic rate was measured as the cell survival rate, then the dose-effect curve was made. LDH leakage rate, axonal length and area of cell body were used as the indicators.</p><p><b>RESULT</b>In 0.125-0.75 g x L(-1) Lishi No. 5 formula could increase the cell survival rate and MTT metabolic rate, decreased LDH leakage rate, and enhance axonal length and area of cell body, compared with control group.</p><p><b>CONCLUSION</b>Lishi No.5 formula has the neurotrophic effect and can induce the survival of neuron.</p>