Effects of vacuum-assisted closure (VAC) on the expressions of MMP-1, 2, 13 in human granulation wound / 中华整形外科杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the changes of metalloproteinases 1, 2, 13 in granulation wound after the treatment of vacuum-assisted closure (VAC).</p><p><b>METHODS</b>The chronic wounds in 5 patients were treated with VAC. The expressions of MMP-1, 2, 13 in the granulation tissues of the chronic wounds were determined and quantified using RT-PCR technique before and at 1, 4, 7 days after the treatment.</p><p><b>RESULTS</b>The MMP-1, 13 mRNA showed obvious decrease, with the steepest variation of MMP-13. The MMP-2 mRNA also showed a decreased tendency, though in an undulatory fashion.</p><p><b>CONCLUSION</b>VAC can promote healing of chronic wounds through depressing the expressions of MMP-1, 2, 13 mRNA and protein synthesis, depressing the degradations of collagen and gelatin.</p>