Effect of hypobaric hypoxic preconditioning on surgically induced endometriosis by allotransplant of uterine tissue in rats / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1369-1376, 2016.
Artigo
em Chinês
| WPRIM
| ID: wpr-256593
ABSTRACT
<p><b>OBJECTIVE</b>To determine the effects of hypobaric hypoxia pretreatment on surgically induced endometriosis in rats.</p><p><b>METHODS</b>Six rats were randomized into 2 groups and exposed to hypoxia (8% O) and normoxia (21% O) for 8 h. The uterine endometrium was intraperitoneally implanted into estrogen-treated ovariectomized Lewis rat, and the growth and quality of the implants were measured. The changes in apoptosis, protein and gene expressions in the serum, abdominis effusion fluids and implants were tested by ELISA, immunohistochemical staining, TUNNEL assay, Western blotting and RT-PCR.</p><p><b>RESULTS</b>The volume of the implants in the hypoxic pretreatment group was significantly increased compared with the normoxia group. High expressions of Ki67, CD31, VEGF, and HIF-1α and lowered cell apoptosis were found in the hypoxia-pretreated implants compared with the normoxic group. VEGF level in the serum and peritoneal fluid were increased in hypoxia-pretreated group, but TNFα level was comparable between the 2 groups.</p><p><b>CONCLUSION</b>Hypoxia play an important role in the occurrence and progression of endometriosis by increasing cell proliferation and angiogenesis and decreasing cell apoptosis in the implants in the rat model.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ratos Endogâmicos Lew
/
Terapêutica
/
Transplante
/
Sangue
/
Líquido Ascítico
/
Western Blotting
/
Apoptose
/
Precondicionamento Isquêmico
/
Fator A de Crescimento do Endotélio Vascular
/
Proliferação de Células
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2016
Tipo de documento:
Artigo
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