Establishment of cryptorchid infertile models by postnatal injection of estradiol in mice / 中华男科学杂志
National Journal of Andrology
;
(12): 19-23, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-256934
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the incidence and spermatogenesis of cryptorchid testes induced by postnatal injection of estradiol.</p><p><b>METHODS</b>Ninety male newborn Balb/C mice were randomly divided into an experimental (n = 60), a solvent control (n = 20) and a normal control group (n = 10). The experimental mice were again assigned to a 4-week, a 6-week, an 8-week, and a 10-week subgroup, and injected subcutaneously with 17-beta estradiol (5 microg/d) from 3 to 28, 3 to 42, 3 to 56 and 3 to 70 days after birth, respectively. The incidence of cryptorchidism and morphological changes of the testes were observed at 2 weeks after drug withdrawal.</p><p><b>RESULTS</b>The incidence rates of cryptorchidism in the 4-, 6-, 8- and 10-week groups were 0%, 26.7%, 60% and 60%, respectively, but no cryptorchidism occurred in the solvent and normal control groups. The 4- and 6-week groups showed autonomous descent of the cryptorchid testes and recovery of spermatogenesis after drug withdrawal. The models became stable and no spermatogenesis recovery was observed after 8 weeks of continuous medication.</p><p><b>CONCLUSION</b>Stable cryptorchid infertile models can be established in mice by postnatally continuous injection of estradiol for over 8 weeks.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Criptorquidismo
/
Modelos Animais de Doenças
/
Estradiol
/
Infertilidade Masculina
/
Animais Recém-Nascidos
/
Camundongos Endogâmicos BALB C
Limite:
Animais
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2013
Tipo de documento:
Artigo
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