Impact of aging on the secretion of insulin-like growth factor-1 in the corpus cavernosum smooth muscle cells of rats in vitro / 中华男科学杂志
National Journal of Andrology
;
(12): 6-9, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-256937
ABSTRACT
<p><b>OBJECTIVE</b>To observe the secretion of insulin-like growth factor-1 (IGF-1) in corpus cavernosum smooth muscle cells (CCSMCs) in rats of different ages and explore the possible relationship of IGF-1 with aging-related erectile dysfunction (ED).</p><p><b>METHODS</b>We primarily cultured CCSMCs of rats aged 4, 12 and 24 months, and identified them by immunohistochemistry. We quantitatively cultured the CCSMCs in 6-well culture plates, determined the levels of IGF-1 secreted from the CCSMCs by enzyme immunoassay (EIA) and analyzed the effect of age on the IGF-1 level.</p><p><b>RESULTS</b>CCSMCs were successfully cultured in vitro. The level of IGF-1 secreted from the CCSMCs was decreased with the increase of age, with 7.1 ng/10(5) cells in the 4-month-old group, 2.2 ng/10(5) cells in the 12-month group, and 1.9 ng/10(5) cells in the 24-month group, with statistically significant differences among the three groups (P < 0.01).</p><p><b>CONCLUSION</b>The secretion of IGF-1 is reduced with the increase of age, and the decreased expression of IGF-1 might be associated with aging-related ED.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pênis
/
Envelhecimento
/
Fator de Crescimento Insulin-Like I
/
Células Cultivadas
/
Ratos Sprague-Dawley
/
Secreções Corporais
/
Biologia Celular
/
Miócitos de Músculo Liso
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2013
Tipo de documento:
Artigo
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