Application of TRUST, TP-ELISA and TPPA in the serologic diagnosis of syphilis / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the application value of toluidine red unheated serum test (TRUST), treponema pallidum enzyme-linked immunosorbent assay (TP-ELISA) and treponema pallidum particle agglutination assay (TPPA) in the serologic diagnosis of syphilis.</p><p><b>METHODS</b>We collected serum samples from 12 622 inpatients, 2 253 outpatients and 1 500 subjects in the medical examination center, and screened them by TRUST and TP-ELISA, followed by quantitative tests using TRUST and TPPA.</p><p><b>RESULTS</b>Among the 16 375 cases, clinically diagnosed syphilis was confirmed in 384, with a positive rate of 100% either by TRUST (12 -116) or TPPA (180 - 12 560), and 92.2% by TP-ELISA.</p><p><b>CONCLUSION</b>TP-ELISA and TRUST should be simultaneously used for syphilis screening, but not TP-ELISA alone, and the results should be confirmed by TPPA. The combination of the three methods plays a valuable role in reducing the misdiagnosis and missed diagnosis, and contributes to the early and accurate diagnosis of syphilis.</p>