Effects of SUMO specific protease 1 on hPXR-mediated P-gp gene expression / 药学学报
Acta Pharmaceutica Sinica
;
(12): 1192-1196, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-257007
ABSTRACT
The study aimed to investigate the effects of small ubiquitin-related modifier (SUMO) specific protease 1 (SENP1) on human PXR-mediated MDR1 transcriptional activity and mRNA expression. Empty vector and expression plasmids, including PXR, SENP1 and SENP1 mutant (SENP1m) were transiently transfected into HepG2 and LS174T cells using Lipo2000. Transcriptional activity was detected by dual luciferase reporter gene assay, and mRNA level was measured using real-time polymerase chain reaction. The results showed that SENP1 could remarkably reduce the rifampicin (RIF)-induced MDR1 reporter activity and mRNA level in hPXR over expressed HepG2 and LS174T cells (P < 0.05), whereas adding SENP1m restored the RIF-induced increases (P < 0.05). These results indicated that SENP1 could repress the RIF-induced hPXR-mediated MDR1 transcriptional activity and mRNA expression.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Endopeptidases
/
RNA Mensageiro
/
Cisteína Endopeptidases
/
Expressão Gênica
/
Ativação Transcricional
/
Receptores Citoplasmáticos e Nucleares
/
Subfamília B de Transportador de Cassetes de Ligação de ATP
/
Células Hep G2
/
Receptor 1 de Sinal de Orientação para Peroxissomos
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2015
Tipo de documento:
Artigo
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