The mechanisms of heparin-derived oligosaccharide on the inhibition of smooth muscle cells proliferation induced by platelet-derived growth factor / 药学学报
Acta Pharmaceutica Sinica
; (12): 993-999, 2015.
Article
em Zh
| WPRIM
| ID: wpr-257036
Biblioteca responsável:
WPRO
ABSTRACT
In this study, the effect of heparin-derived oligosaccharide (HDO) on platelet-derived growth factor (PDGF) induced vascular smooth muscle cells (VSMCs) proliferation and the related signal transduction mechanisms were investigated. MTT assays were used to measure VSMCs proliferation. Cell cycle distribution was analyzed by flow cytometry. The level of key regulatory proteins in PKC, MAPK and Akt/PI3K pathways were determined by RT-PCR, Western blot and immunocytochemical methods. Meanwhile, mRNA expressions of some proto-oncogenes were assayed by RT-PCR method. Our data showed that HDO (0.01, 0.1 and 1 μmol · L(-1)) inhibited 30 ng · mL(-1) PDGF-induced VSMCs proliferation in a dose-dependent manner, blocked the G1/S transition and inhibited the level of key regulatory proteins and some proto-oncogenes (P < 0.05). The results showed that HDO may decrease the key regulatory proteins expression, hence suppress the transcription of proto-oncogene and G1/S transition, finally inhibiting VSMCs proliferation.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Oligossacarídeos
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Farmacologia
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Fator de Crescimento Derivado de Plaquetas
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Heparina
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Transdução de Sinais
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Ciclo Celular
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Células Cultivadas
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Biologia Celular
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Miócitos de Músculo Liso
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Proliferação de Células
Limite:
Humans
Idioma:
Zh
Revista:
Acta Pharmaceutica Sinica
Ano de publicação:
2015
Tipo de documento:
Article