Protecting action of acupuncture serum on calcium over-loaded neurons of the hippocampus in rats of ischemia-reperfusion / 中国针灸
Chinese Acupuncture & Moxibustion
;
(12): 45-47, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-257135
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of acupuncture serum on Ca2+ content in the cultured nervous cells of hippocampus after ischemia-reperfusion, so as to probe into humoral factors in acupuncture treatment.</p><p><b>METHODS</b>The neurons of the hippocampus from the new born rats were cultured for 9-11 days. Fluorescein-molecular probe Fluo-3 AM was used for staining of intracellular Ca2+. Fluorescent levels in the nervous cells cultured with the serum of the normal rats or the rats given electroacupuncture at "Baihui" (GV 20), "Zusanli" (ST 36), "Quchi" (LI 11) and "Sanyinjiao" (SP 6) for 2 weeks were determined by using a laser confocal microscope.</p><p><b>RESULTS</b>After the normal serum was added, the intracellular Ca2+ fluorescent levels increased to 697 +/- 113 from 461 +/- 96, while after acupuncture serum was added, the Ca2+ fluorescent levels decreased to 584 +/- 103 from 673 +/- 108, indicating that after addition of acupuncture serum, the increased intracellular Ca2+ content could be decreased.</p><p><b>CONCLUSION</b>There are some active substances in acupuncture serum which can obviously decrease intracellular Ca2+ content after ischemia-reperfusion, so as to provide a direct evidence for role of humoral factor in acupuncture treatment.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Terapêutica
/
Proteínas Sanguíneas
/
Traumatismo por Reperfusão
/
Distribuição Aleatória
/
Terapia por Acupuntura
/
Células Cultivadas
/
Isquemia Encefálica
/
Química
/
Cálcio
Tipo de estudo:
Ensaio Clínico Controlado
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Acupuncture & Moxibustion
Ano de publicação:
2009
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS