Effect of Intestinal Ischemia-reperfusion Injury on the Expression of Chemerin in Mice / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 440-445, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-257614
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of intestinal ischemia-reperfusion on the protein expression of chemerin in C57BL/6J mice.</p><p><b>METHODS</b>A total of 16 mice (C57BL/6J, specific pathogen free level) were randomly assigned into two groups (n=8 each) the intestinal ischemia-reperfusion group and the sham group. The intestinal ischemia-reperfusion state was achieved by blocking the super-mesenteric artery. After 60 minutes of ischemia followed by 60 minutes of reperfusion,we determined the protein level of chemerin in various organs and tissues by enzyme-linked immunosorbent assay and Western blot. The sham group underwent the same operation process except for the blocking of the super-mesenteric artery.</p><p><b>RESULT</b>The protein level of chemerin was significantly elevated in distinctive organs and tissues in the state of intestinal ischemia-reperfusion (P<0.05).</p><p><b>CONCLUSION</b>The intestinal ischemia-reperfusion can remarkably increase the protein expression of chemerin in some organs and tissues.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ferimentos e Lesões
/
Ensaio de Imunoadsorção Enzimática
/
Traumatismo por Reperfusão
/
Quimiocinas
/
Peptídeos e Proteínas de Sinalização Intercelular
/
Intestinos
/
Isquemia
/
Camundongos Endogâmicos C57BL
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2015
Tipo de documento:
Artigo
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