Evaluation of HER2 gene expression status in breast cancer by fluorescence in-situ hybridization / 中华病理学杂志
Chinese Journal of Pathology
;
(12): 701-705, 2005.
Artigo
em Chinês
| WPRIM
| ID: wpr-258288
ABSTRACT
<p><b>OBJECTIVE</b>To deduce the protocol, scoring criteria and interpretive guidelines for assessment of HER2 gene expression status by fluorescence in-situ hybridization (FISH) and to compare the results with those obtained by immunohistochemistry.</p><p><b>METHODS</b>The HercepTest kit from Dako Cytomation was employed for immunohistochemistry. FISH for HER2 gene expression status was performed using PathVysion DNA probe kit on the archival paraffin-embedded sections of breast cancer tissues from 28 Chinese female patients with immunohistochemical staining scores of (3 +), (2 +), (1 +) and 0.</p><p><b>RESULTS</b>Ten of the 12 patients with score (3 +) by immunohistochemistry were positive for HER2 by FISH, with 2 cases being polysomy. Two other cases with FISH-negative were also shown to be polysomy. Seven of the 10 patients with score (2 +) by immunohistochemistry showed HER2 gene amplification, with 1 case being polysomy. Two of the remaining 3 cases, which were FISH-negative, were shown to be polysomy. All the patients with scores (1 +, number = 3 ) or 0 ( number = 3) by immunohistochemistry failed to show amplification. One case of polysomy was noted in either group.</p><p><b>CONCLUSIONS</b>Immunohistochemistry is useful as an initial screening tool for HER2 expression status. Because of the obvious discrepancies between protein expression and gene amplification, patients with score (2 +) by immunohistochemistry should undergo FISH testing as well. FISH is also required in selected examples with score (3 +) immunohistochemical results, especially in those with false-positive immunohistochemistry due to chromosome 17 aneuploidy.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Poliploidia
/
Cromossomos Humanos Par 17
/
Neoplasias da Mama
/
Imuno-Histoquímica
/
Regulação Neoplásica da Expressão Gênica
/
Amplificação de Genes
/
Hibridização in Situ Fluorescente
/
Carcinoma Ductal de Mama
/
Receptor ErbB-2
/
Genes erbB-2
Limite:
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Pathology
Ano de publicação:
2005
Tipo de documento:
Artigo
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