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The Cellular Toxicity of PM2.5 Emitted from Coal Combustion in Human Umbilical Vein Endothelial Cells / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 107-116, 2016.
Artigo em Inglês | WPRIM | ID: wpr-258846
ABSTRACT
<p><b>OBJECTIVE</b>To explore the relationship between different components of fine particulate matter (PM2.5) emitted from coal combustion and their cytotoxic effect in the vascular endothelial cells.</p><p><b>METHODS</b>Coal-fired PM(2.5) was sampled using a fixed-source dilution channel and flow sampler. The sample components were analyzed by ion chromatography and inductively coupled plasma atomic emission spectroscopy (ICP-AES). The PM(2.5) suspension was extracted using an ultrasonic water-bath method and then human umbilical vein endothelial cells (EA.hy926) were treated with various concentrations of the PM(2.5) suspension. Cell proliferation, oxidative DNA damage, and global DNA methylation levels were used to measure the cellular toxicity of PM(2.5) emitted from coal combustion.</p><p><b>RESULTS</b>Compared to other types of coal-fired PM(2.5) preparations, the PM2.5 suspension from Yinchuan coal had the highest cytotoxicity. PM(2.5) suspension from Datong coal had the highest toxic effect while that from Yinchuan coal had the lowest. Exposure to coal-fired PM(2.5) from Jingxi coal resulted in lower 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels. At the same dose, PM(2.5) emitted from coal combustion could produce more severe DNA impairment compared to that produced by carbon black. Cell survival rate was negatively correlated with chloride and potassium ions content. The 5-methylcytosine (5-mC) level was positively correlated with Mn and negatively correlated with Zn levels. The 8 OHdG% level was positively correlated with both Mn and Fe.</p><p><b>CONCLUSION</b>PM(2.5) emitted from coal combustion can decrease cell viability, increase global DNA methylation, and cause oxidative DNA damage in EA.hy926 cells. Metal components may be important factors that influence cellular toxicity.</p>
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Dano ao DNA / Testes de Toxicidade / Metilação de DNA / Proliferação de Células / Toxicidade / Células Endoteliais da Veia Umbilical Humana / Cinza de Carvão Idioma: Inglês Revista: Biomedical and Environmental Sciences Ano de publicação: 2016 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Dano ao DNA / Testes de Toxicidade / Metilação de DNA / Proliferação de Células / Toxicidade / Células Endoteliais da Veia Umbilical Humana / Cinza de Carvão Idioma: Inglês Revista: Biomedical and Environmental Sciences Ano de publicação: 2016 Tipo de documento: Artigo