Your browser doesn't support javascript.
loading
Identification of Variable-Number Tandem-Repeat (VNTR) Sequences in Acinetobacter pittii and Development of an Optimized Multiple-Locus VNTR Analysis Typing Scheme / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences ; (12): 855-863, 2015.
Artigo em Inglês | WPRIM | ID: wpr-258867
ABSTRACT
<p><b>OBJECTIVE</b>To develop a multiple-locus variable-number tandem-repeat (VNTR) analysis (MLVA) assay for Acinetobacter pittii typing.</p><p><b>METHODS</b>Polymorphic VNTRs were searched by Tandem Repeats Finder. The distribution and polymorphism of each VNTR locus were analyzed in all the A. pittii genomes deposited in the NCBI genome database by BLAST and were evaluated with a collection of 20 well-characterized clinical A. pittii strains and one reference strain. The MLVA assay was compared with pulsed-field gel electrophoresis (PFGE) for discriminating A. pittii isolates.</p><p><b>RESULTS</b>Ten VNTR loci were identified upon bioinformatic screening of A. pittii genomes, but only five of them showed full amplifiability and good polymorphism. Therefore, an MLVA assay composed of five VNTR loci was developed. The typeability, reproducibility, stability, discriminatory power, and epidemiological concordance were excellent. Compared with PFGE, the new optimized MLVA typing scheme provided the same and even greater discrimination.</p><p><b>CONCLUSION</b>Compared with PFGE, MLVA typing is a faster and more standardized alternative for studying the genetic relatedness of A. pittii isolates in disease surveillance and outbreak investigation.</p>
Assuntos

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Acinetobacter / Reação em Cadeia da Polimerase / Impressões Digitais de DNA / Classificação / Eletroforese em Gel de Campo Pulsado / Repetições Minissatélites / Genética / Métodos Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Idioma: Inglês Revista: Biomedical and Environmental Sciences Ano de publicação: 2015 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Acinetobacter / Reação em Cadeia da Polimerase / Impressões Digitais de DNA / Classificação / Eletroforese em Gel de Campo Pulsado / Repetições Minissatélites / Genética / Métodos Tipo de estudo: Estudo diagnóstico / Estudo prognóstico Idioma: Inglês Revista: Biomedical and Environmental Sciences Ano de publicação: 2015 Tipo de documento: Artigo