Gene expression profile of isoniazid liver-injured rat using cDNA microarray / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 308-314, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-259022
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the changes of gene expression profile of rat liver tissue by cDNA microarrays.</p><p><b>METHODS</b>Twenty Wistar rats in control group (n = 10) and isoniazid (INH) group (n = 10) were orally administrated with normal saline and 400 mg/kg INH for 14 days, respectively. The differentially expressed genes significantly correlated with liver injury were screened and analyzed. The mechanisms of liver injury caused by INH were specifically analyzed at level of gene expression based on the biological functions of those differentially expressed genes.</p><p><b>RESULTS</b>Thirty-seven differentially expressed genes were found in the rats administrated with INH. Among them, 25 genes were up-regulated, while the other 12 genes were down-regulated. These differentially expressed genes were functionally related to the changes of CYP450-related genes, fatty acid metabolism, and protein metabolism.</p><p><b>CONCLUSION</b>INH can cause the remarkable changes of the gene expression profiles in rat liver cells, which is important for further elucidating the mechanisms of liver injury caused by INH.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Ratos Wistar
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Perfilação da Expressão Gênica
/
Modelos Animais de Doenças
/
Toxicidade
/
Doença Hepática Induzida por Substâncias e Drogas
/
Transcriptoma
/
Isoniazida
/
Fígado
Limite:
Animais
Idioma:
Chinês
Revista:
Acta Academiae Medicinae Sinicae
Ano de publicação:
2009
Tipo de documento:
Artigo
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