Expression of phytase gene in Bombyx mori / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 112-115, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-259184
ABSTRACT
Phytase gene of Aspergillus niger 963 was cloned into baculovirus transfer vector. DNA of the recombinant vector was co-transfected with Bm-BacPAK6 DNA into BmN cells, and recombinant virus was selected by plaque assays. The recombinant virus was identified by Dot blot and Southern-blot with the specific probe for phytase gene. Phytase gene was expressed in silkworm larvae and pupae. The expression product was 1.43 g/L haemolymph for silkworm larvae and 1.90 g/L haemolymph for pupae, respectively. The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied in this paper.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Aspergillus niger
/
Bombyx
/
Proteínas Fúngicas
/
Southern Blotting
/
6-Fitase
/
Eletroforese em Gel de Poliacrilamida
/
Vetores Genéticos
/
Genética
/
Concentração de Íons de Hidrogênio
/
Metabolismo
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2003
Tipo de documento:
Artigo
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