Expression of phytase gene in Bombyx mori / 生物工程学报
Chinese Journal of Biotechnology
; (12): 112-115, 2003.
Article
em Zh
| WPRIM
| ID: wpr-259184
Biblioteca responsável:
WPRO
ABSTRACT
Phytase gene of Aspergillus niger 963 was cloned into baculovirus transfer vector. DNA of the recombinant vector was co-transfected with Bm-BacPAK6 DNA into BmN cells, and recombinant virus was selected by plaque assays. The recombinant virus was identified by Dot blot and Southern-blot with the specific probe for phytase gene. Phytase gene was expressed in silkworm larvae and pupae. The expression product was 1.43 g/L haemolymph for silkworm larvae and 1.90 g/L haemolymph for pupae, respectively. The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied in this paper.
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Aspergillus niger
/
Bombyx
/
Proteínas Fúngicas
/
Southern Blotting
/
6-Fitase
/
Eletroforese em Gel de Poliacrilamida
/
Vetores Genéticos
/
Genética
/
Concentração de Íons de Hidrogênio
/
Metabolismo
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2003
Tipo de documento:
Article