Differences of acetylcholinesterase level in variety classes and strains of Culex pipiens pallens / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 511-514, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-259274
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the diversity of acetylcholinesterase (AChE) activity in variety classes and strains of Culex pipiens pallens and provide a basis for the insecticide-resistance detection of mosquito by biochemical method.</p><p><b>METHODS</b>AChE insensitivity of single mosquito was determined, using acetythiocholine iodide (ATch) as the substrate, 5,5'-dithio-bis (2-nitrobenzoic acid) (DTNB) as the developer, and propoxur as the inhibitor.</p><p><b>RESULT</b>There were significant differences in AChE activity among the four types of IV instar larvae and 3-day-old adult female of sensitive strain mosquito (P<0.01). The AChE activity of the 3-day-old adult female was higher than that of IV instar larvae of the four types of sensitive strain mosquito (P<0.01). The AChE activity of anti-DDVP (Rd) and anti-propoxur (Rp) strains of Culex pipiens pallens was significantly higher than that of sensitive (S) strain (P<0.01), while the AChE activity of anti-cypermethrin (Rc) strain of Culex pipiens pallens was similar to that of S strain (P>0.05). The individual frequency of insensitive AChE of Rd and Rp strains of Culex pipiens pallens was significantly higher than that of sensitive (S) strain (P<0.01), while the individual frequency of insensitive AChE of Rc strain of Culex pipiens pallens was similar to that of S strain(P>0.05).</p><p><b>CONCLUSION</b>The AChE activity determination can be used to examine the insecticide-resistance of mosquito.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Propoxur
/
Acetilcolinesterase
/
Piretrinas
/
Especificidade da Espécie
/
Resistência a Inseticidas
/
Classificação
/
Culex
/
Diclorvós
/
Metabolismo
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2009
Tipo de documento:
Artigo
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