Analysis of serum metabonome of patients with breast cancer by gas chromatography-mass spectrometry / 浙江大学学报·医学版

ABSTRACT

<p><b>OBJECTIVE</b>To analyze serum metabonome of patients with breast carcinoma by gas chromatography-mass spectrometry (GC-MS).</p><p><b>METHODS</b>Serum samples from 30 patients with breast carcinoma and 25 normal people were collected. Endogenous metabolites amino acids, fatty acids, saccharides and organic acids were analyzed after derivatization.</p><p><b>RESULT</b>The injection precision and intra-day precision (RSD) were less than 14.4 %. The method displayed good recovery (80.7 %-118.0 % with RSD<12.0 %) and linearity (r(2)>0.9905). Samples were stable in 24 h at room temperature. The samples were stable through three freeze-thaw cycles (RSD<14.6 %). Alaine,oxalate and glutamine were found with frequency of 96.7 %, 100 % and 93.3 %, respectively, which indicated that they may be the potential biomarkers.</p><p><b>CONCLUSION</b>The method is stable and reliable, which may be helpful for the diagnosis of breast cancer as an auxiliary method.</p>