Research progress of probe design software of oligonucleotide microarrays / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 214-221, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-259667
ABSTRACT
DNA microarray has become an essential medical genetic diagnostic tool for its high-throughput, miniaturization and automation. The design and selection of oligonucleotide probes are critical for preparing gene chips with high quality. Several sets of probe design software have been developed and are available to perform this work now. Every set of the software aims to different target sequences and shows different advantages and limitations. In this article, the research and development of these sets of software are reviewed in line with three main criteria, including specificity, sensitivity and melting temperature (Tm). In addition, based on the experimental results from literatures, these sets of software are classified according to their applications. This review will be helpful for users to choose an appropriate probe-design software. It will also reduce the costs of microarrays, improve the application efficiency of microarrays, and promote both the research and development (R&D) and commercialization of high-performance probe design software.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Design de Software
/
Software
/
Sequência de Bases
/
Sondas de Oligonucleotídeos
/
Sensibilidade e Especificidade
/
Análise de Sequência com Séries de Oligonucleotídeos
Tipo de estudo:
Estudo diagnóstico
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2014
Tipo de documento:
Artigo
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