Preliminary study of chitosan/pcDNA-EGFP-TGFbeta1 nanoparticles used in the transfection of synovial-derived mesenchymal stem cells / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1260-1264, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-259728
ABSTRACT
The objective of this study is to explore the application possibility of chitosan/pcDNA-EGFP-TGFPbeta1 nanoparticles in the transfection of synovial-derived mesenchymal stem cells (SDMSCs). Chitosan/pcDNA-EGFP-TGFbeta1 nanoparticles were fabricated through method of ionic crosslinking. The SDMSCs were harvested from rabbit joints and cultured to passage 3. The SDMSCs were then transfected with chitosan/pcDNA-EGFP-TGFbeta1 nanoparticles. Scanning electronic microscope (SEM) was employed to detect the shape and diameter of the nanoparticles. The transfected SDMSCs were examined under the fluorescence microscope and detected through the flow cytometry (FCM). The SEM examination showed that the contour of the fabricated chitosan/pcDNA-EGFP-TGFbeta1 nanoparticles was round and its average diameter was 50 nm. After being cultured for 48 h, the SDMSCs transfected by chitosan/pcDNA-EGFP-TGFbeta1 nanoparticles could be detected under the fluorescence microscope, and the live SDMSCs could also be examined through FCM. The transfection rate was 8% - 10%. Therefore, it suggested that the chitosan/pcDNA-EGFP-TGFbeta1 nanoparticles fabricated through the method of ionic crosslinking could transfect the SDMSCs, but the transfection rate should be improved.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transfecção
/
Química
/
Biologia Celular
/
Quitosana
/
Proteínas de Fluorescência Verde
/
Microscopia Eletrônica de Transmissão
/
Fator de Crescimento Transformador beta1
/
Nanopartículas
/
Células-Tronco Mesenquimais
/
Vetores Genéticos
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2013
Tipo de documento:
Artigo
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