The anti-HBV effect and mechanism of C gene truncated mutant in vitro / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the effect and mechanism on HBV replication in C gene truncated mutant.</p><p><b>METHODS</b>Protein expression of C gene truncated vector and wild C gene vector were assay by SDS-PAGE Western blot. Constructed C gene truncated expression vector was cotransfected with wild HBV genome; virus load was detected by PCR in the culture medium and the cell. The formation of core particle was assay by Native western blot.</p><p><b>RESULTS</b>The recombinant vectors can efficiently express. Virus load of the cotransfected group by pcDNA3-deltaC and adwR9 was lower than that of control group in the culture medium and the cell. Protein band of the co-expressed group by pcDNA3-deltaC and pcDNA3-C showed slightly weaker than that of the co-expressed group by pcDNA3 and pcDNA3-C.</p><p><b>CONCLUSION</b>C gene truncated mutant could interfere with the formation of core particle and reduce of HBV replication</p>