Preliminary study on proteomic patterns in hepatic tissue to identify HBV related hepatocellular carcinoma / 中华肝脏病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To identify proteomic patterns in hepatic tissues for diagnosing early HBV related HCC.</p><p><b>METHODS</b>Proteomic spectra were generated by two-dimensional gel electrophoresis (2-DE), A preliminary "raining" set of spectra derived from analysis of 14 cancer tissues and 14 non-cancer tissues, a proteomic patterns that completely discriminated cancer from non-cancer was identified. The discovered pattern was then used to classify an independent set of 48 masked samples: 24 from cancer tissues, and 24 from non-cancer tissues.</p><p><b>RESULTS</b>The discriminatory pattern correctly identified all cancer tissues and non-cancer tissues in the masked set. This result yielded a sensitivity of 100%, specificity of 100%.</p><p><b>CONCLUSION</b>Further analysis on these proteins in the proteomic pattern will be helpful to screen tumor markers for HBV related HCC. These findings justify a prospective assessment of proteomic pattern technology as a screening tool for cancer in high-risk and general populations.</p>