Effect of KH2PO4 on the odonto- and osteogenic differentiation potential of human stem cells from apical papillae / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 27-31, 2013.
Article
em Zh
| WPRIM
| ID: wpr-260184
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the effects of KH2PO4 on the odonto- and osteogenic differentiation potential of human stem cells from apical papillae (SCAP) in vitro.</p><p><b>METHODS</b>SCAP were isolated and cultured respectively in alpha minimum essential medium (α-MEM) or α-MEM containing 1.8 mmol/L KH2PO4. Alkaline phosphatase (ALP) activity, alizarin red staining, real-time reverse transcription polymerase chain reaction (RT-PCR) and Western blotting were used to examine the odonto and osteogenic potential of SCAP in the two media.</p><p><b>RESULTS</b>SCAP cultured in α-MEM containing 1.8 mmol/L KH2PO4 exhibited a higher ALP activity [(0.370 ± 0.013) Sigma unit×min(-1)×mg(-1)] at day 3 than control group [(0.285 ± 0.008) Sigma unit×min(-1)×mg(-1)] and KH2PO4-treated SCAP formed more calcified nodules at day 5 [(0.539 ± 0.007) µg/g] and day 7 [(1.617 ± 0.042) µg/g] than those in normal medium [(0.138 ± 0.037) µg/g, P < 0.01]. The expression of odonto- and osteogenic markers were significantly up-regulated after the stimulation of KH2PO4 at day 3 and 7 respectively, as compared with control group.</p><p><b>CONCLUSIONS</b>1.8 mmol/L KH2PO4 can promote the odonto and osteogenic differentiation potential of human SCAP.</p>
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Assunto principal:
Osteoblastos
/
Farmacologia
/
Fosfatos
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Fosfoproteínas
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Sialoglicoproteínas
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Células-Tronco
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Osteocalcina
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Diferenciação Celular
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Células Cultivadas
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Proteínas da Matriz Extracelular
Limite:
Humans
Idioma:
Zh
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2013
Tipo de documento:
Article