Construction of recombinant plasmid with Porphyromonas gingivalis FimA deficiency / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 671-674, 2012.
Article
em Zh
| WPRIM
| ID: wpr-260212
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To construct the recombinant plasmid pPHU281_A_Spec_B, which knock out Porphyrmonas gingivalis (Pg) FimA gene.</p><p><b>METHODS</b>Genomic DNA was extracted from PgATCC33277 which was cultured in anaerobic condition. The upstream and downstream gene of FimA was cloned from Pg genenomic DNA with specific restriction sites by polymerase chain reaction. Suicide vector pPHU281 was inserted by three fragments: upstream, downstream of FimA gene and spectinomycin resistance gene. The recombinant plasmid was confirmed by electrophoresis and sequenced after amplification in compentent cells DH-5α.</p><p><b>RESULTS</b>The gene sequence was identified by DNA sequencing analysis. The recombinant plasmid pPHU281_A_Spec_B was successfully constructed.</p><p><b>CONCLUSIONS</b>The recombinant plasmid pPHU281_A_Spec_B was constructed, which may be used for the constructon of FimA deficient Pg.</p>
Texto completo:
1
Índice:
WPRIM
Assunto principal:
Plasmídeos
/
Proteínas Recombinantes
/
DNA Bacteriano
/
Sequência de Bases
/
Análise de Sequência de DNA
/
Porphyromonas gingivalis
/
Proteínas de Fímbrias
/
Técnicas de Inativação de Genes
/
Genes Bacterianos
/
Vetores Genéticos
Idioma:
Zh
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2012
Tipo de documento:
Article