Effect of fish oil on intestinal Paneth cells in mouse with abdominal infection / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 702-706, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-260281
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of fish oil on intestinal Paneth cells in mouse with abdominal infection.</p><p><b>METHODS</b>Fifty C57BL/6J mouse were randomly divided into five groups (n=10 each) control group, sham group, infection group (cecal ligation and puncture, CLP), fish oil group (0.4 g/kg fish oil, intragastric administration every day, FO) and long chain triglyceride group (0.4 g/kg soybean oil, intragastric administration every day, LCT). The mouse were sacrificed and the terminal ileum was collected for lysozyme, cryptdin 4 and secreted phosphatidase A2 (sPLA2) analysis at the fourth day after operation. The changes of mouse body weight and intestinal mucosa pathology were observed.</p><p><b>RESULTS</b>The body weight, the mRNA levels of lysozyme, cryptdin 4 and sPLA2 and the protein level of lysozyme of Paneth cells in the infection group were reduced compared with the control group (0.78±0.34 vs. 1.83±0.11, 0.99±0.44 vs. 2.02±0.33, 0.92±0.25 vs. 1.50±0.27, 0.31±0.06 vs. 0.45±0.05, all P<0.05), meanwhile the intestinal villi collapse and breakage occurred obviously. Fish oil could up-regulate the mRNA and protein expression of lysozyme (1.23±0.27 vs. 0.78±0.34 and 0.62±0.23, 0.38±0.07 vs. 0.31±0.06 and 0.32±0.06, all P<0.05) and alleviate the mucosa injury compared with the infection group and LCT group.</p><p><b>CONCLUSIONS</b>The function of intestinal Paneth cells is damaged apparently after cecal ligation and puncture. Fish oil can relieve this injury.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Óleos de Peixe
/
Ceco
/
Regulação para Cima
/
Celulas de Paneth
/
Infecções Intra-Abdominais
/
Mucosa Intestinal
/
Intestino Delgado
/
Camundongos Endogâmicos C57BL
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Gastrointestinal Surgery
Ano de publicação:
2015
Tipo de documento:
Artigo
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