Impact of lysosome-associated protein transmembrane-4 beta on proliferation and invasion of colorectal cancer / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
;
(12): 606-610, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-260301
ABSTRACT
<p><b>OBJECTIVE</b>To determine whether lysosome-associated protein transmembrane-4 beta (LAPTM4B) over-expression is associated with the proliferation and invasion in colorectal cancer (CRC).</p><p><b>METHODS</b>Thirty pairs of CRC tissues, containing carcinoma and adjacent tissues, were used for the examination of LAPTM4B mRNA expression by real-time quantitative PCR (qPCR) assays. Then immunohistochemistry was performed to examine LAPTM4B protein expression in 6 pairs of CRC tissues. Over-expression LAPTM4B and low-expression LAPTM4B cell models were constructed with HCT116 CRC cell lines. CCK8 assay was used to detect the proliferation and Transwell assay was used to detect the invasion of the model cells.</p><p><b>RESULTS</b>qPCR and immunohistochemistry results showed that LAPTM4B expression levels in CRC were higher compared to adjacent tissues (all P<0.01). CCK8 and Transwell assays results showed that LAPTM4B promoted proliferation and invasion of HCT116 cell lines model cells (all P<0.01).</p><p><b>CONCLUSION</b>LAPTM4B promotes the proliferation and invasion in CRC patients, and may be used as an important potential marker.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Imuno-Histoquímica
/
Neoplasias Colorretais
/
Proteínas Oncogênicas
/
Células HCT116
/
Proliferação de Células
/
Proteínas de Membrana
/
Invasividade Neoplásica
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Gastrointestinal Surgery
Ano de publicação:
2015
Tipo de documento:
Artigo
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