A study on the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 735-737, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-260612
ABSTRACT
<p><b>OBJECTIVE</b>To study the relativity between La protein and the stability of HBV mRNA and the expression of HBV protein.</p><p><b>METHODS</b>Four specific siRNAs were obtained by transcription in vitro. After transfection with the siRNAs into HepG2.2.15 cells for 3 days, the inhibitive effects of La protein were analyzed by Western blot; the content changes of HBsAg, HBeAg and HBV-DNA were detected by ECL and RT-PCR.</p><p><b>RESULTS</b>In comparison to normal cells, La protein was less in the cells. There was less La protein in the cells trans-infected with siRNAs. HBsAg, the HBeAg and HBV-DNA secreted by the cells transfected with siRNA were also less than that in the normal cells.</p><p><b>CONCLUSION</b>There is a correlation between La protein and HBV mRNA and the expression of HBV protein.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Ribonucleoproteínas
/
Autoantígenos
/
DNA Viral
/
RNA Mensageiro
/
RNA Viral
/
Vírus da Hepatite B
/
RNA Interferente Pequeno
/
Linhagem Celular Tumoral
/
Genética
/
Antígenos de Superfície da Hepatite B
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hepatology
Ano de publicação:
2006
Tipo de documento:
Artigo
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