Characterizing effects of solvent specific morus alba components on rat platelet aggregation, vascular tension and macrophage nitrite production / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 3024-3028, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-260695
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of different solvent extractions of Mori Ramulus on platelet aggregation, vascular tension, and nitrite production from macrophages stimulated by lipopolysaccharides and interferon-gamma.</p><p><b>METHOD</b>The components of Mori Ramulus were extracted by EtoAc, n-BuOH and chloroform respectively. Platelet aggregation was induced by ADP, arachidonic acid and collagen in vitro; nitrite production of activated macrophages was measured by Griess assay, and the vasodilatory effects of three extractions were investigated by isometric tension changes of aortic rings.</p><p><b>RESULT</b>Chloroform extraction concentration-dependently inhibited platelet aggregation by arachidonic acid, and reduced vascular tension of PE preconstricted aorta rings with or without endothelium. On the other hand, extractions of EtoAc and n-BuOH demonstrated dose-dependent inhibition on macrophage NO production stimulated by LPS/IFN-gamma.</p><p><b>CONCLUSION</b>Pharmacological activities of Mori Ramulus depend on solvent specific components. Chloroform extraction of Mori Ramulus may benefit cardiovascular diseases through its properties of anti-platelet aggregation and vasodilatation. The inhibition of macrophage activity by EtoAc and n-BuOH extractions suggested an anti-inflammation effect of the compound.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Aorta
/
Farmacologia
/
Vasodilatação
/
Vasodilatadores
/
Técnicas In Vitro
/
Medicamentos de Ervas Chinesas
/
Inibidores da Agregação Plaquetária
/
Linhagem Celular
/
Química
/
Agregação Plaquetária
Limite:
Animais
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2010
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS