Effects of different fibrin glue combination modes on the proliferation and viability of dental follicle cells / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 135-140, 2015.
Artigo
em Chinês
| WPRIM
| ID: wpr-261120
ABSTRACT
<p><b>OBJECTIVE</b>This study explores the effects of different fibrin glue combination modes on the survival, proliferation, and apoptosis of dental follicle cells (DFCs), as well as to evaluate the feasibility and effectiveness of fibrin glue as transplantation material.</p><p><b>METHODS</b>The membranes of surviving DFCs were marked using 3,3'-dioctadecyloxa carbocyanine perchlorate (DIO), and the cell number was counted by using ImageJ2x software. The apoptotic cells were marked with prodium iodide (PI).</p><p><b>RESULTS</b>Compared with that of the 3D-2 and 2D-1 groups, the degradation speed of the 3D-1 group was the slowest. DFCs could survive and grow well in fibrinogen with a concentration of 15 mg · mL⁻¹ supplemented with thrombin with a concentration of 2 U · mL⁻¹. In particular, the 3D-1 combination mode was significantly conducive to cell proliferation and stretching.</p><p><b>CONCLUSION</b>Fibrin glue can be used as an effective cell transplantation material. The different combination modes have certain effects on cell proliferation. The 3D-1 combination mode is more conducive to the survival and proliferation of DFCs than other modes.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fibrinogênio
/
Trombina
/
Adesivo Tecidual de Fibrina
/
Sobrevivência Celular
/
Apoptose
/
Biologia Celular
/
Saco Dentário
/
Proliferação de Células
Limite:
Humanos
Idioma:
Chinês
Revista:
West China Journal of Stomatology
Ano de publicação:
2015
Tipo de documento:
Artigo
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