Effects of bcl-2 antisense oligodeoxynucleotide on proliferation and apoptosis of Raji cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 71-73, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-261358
ABSTRACT
<p><b>OBJECTIVE</b>To study the in vitro antitumor activity of bcl-2 fully phosporothioated antisense oligodeoxynucleotide (bcl-2 ASODN) to malignant lymphoblastic cells.</p><p><b>METHODS</b>Proliferation and apoptosis of Raji cells incubated with bcl-2 ASODN were evaluated by MTT assay, flow cytometry (FCM) and electron microscopy, and the level of bcl-2 protein and mRNA expression were assessed by FCM and RT-PCR, respectively.</p><p><b>RESULTS</b>MTT assay demonstrated that bcl-2 ASODN could partially inhibit the growth of Raji cells. After incubated with ASODN for 48 hours, Raji cells exhibited characteristic morphologic changes of apoptosis, including cytoplasm membrane blebbing, chromatin condensation crescents formation and nuclear fragmentation. The apoptosis rate of Raji cells treated with 20 micromol/L bcl-2 ASON for 72 hrs was 43.86% which is significantly higher than that of control (10.05%). The bcl-2 ASODN induced apoptosis of Raji cells was accompanied by declined expression of bcl-2 mRNA, which decreased to 0.88% at 72 hrs and was significantly lower than that of control (79.54%).</p><p><b>CONCLUSION</b>bcl-2 ASODN induced Raji cells apoptosis by downregulating bcl-2 protein.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
RNA Mensageiro
/
Células Tumorais Cultivadas
/
Regulação Neoplásica da Expressão Gênica
/
Divisão Celular
/
Oligonucleotídeos Antissenso
/
Apoptose
/
Proteínas Proto-Oncogênicas c-bcl-2
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Relação Dose-Resposta a Droga
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2003
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS