Identification of multidrug resistance related genes in leukemia by suppression subtractive hybridization / 中华血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To clone and screen genes related to multidrug resistance (MDR) in leukemia.</p><p><b>METHODS</b>Suppression subtractive hybridization (SSH) was performed to profile differentially expressed genes between a MDR leukemia cell line (K562/DOX, as tester) and its parent cell line (K562, as driver). Reverse Northern dot blot was carried out to further screen the subtracted cDNA library. The overexpressed cDNA fragments in K562/DOX cells were sequenced and compared with known genes in Genbank. RT-PCR and Northern blot were employed to confirm the differential expression of some identified genes.</p><p><b>RESULTS</b>Eleven genes were identified being overexpressed in K562/DOX, including S3 ribosomal protein (S3rp) gene, NADH dehydrogenase subunit 2 (ND2) gene and My023 gene, which have not been reported to be related to MDR in cancer.</p><p><b>CONCLUSION</b>Several genes, which might be involved in MDR were identified, indicating novel mechanisms of MDR in leukemia.</p>