Analysis and identification of transcriptional repression domain of ETO / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 10-13, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-261368
ABSTRACT
<p><b>OBJECTIVE</b>To further verify the transcriptional repression domains in ETO and their relationship with histone deacetylase (HDAC).</p><p><b>METHODS</b>Either of the ETO two zinc fingers was mutated respectively by site-directing mutagenesis. The truncation fragments of ETO were amplified by polymerase chain reaction (PCR) and cloned into eukaryotic expression plasmid pFA-CMV. By the means of DNA transfection and analysis of the transcription derived from the promoter of reporter gene, the transcriptional regulation domains of ETO was determined.</p><p><b>RESULTS</b>The expression plasmids carrying truncated ETO and ETO with point mutation at either zinc finger were successfully constructed. Two repression domains were found within ETO, which were located at two zinc finger motifs and 275 - 487 amino acid residues, respectively.</p><p><b>CONCLUSION</b>The transcription repression by ETO was mediated by two separated domains and closely associated with HDAC, which may be used as therapeutic target for acute myeloid leukemia M(2b).</p>
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DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Transcrição Gênica
/
Técnicas In Vitro
/
Transfecção
/
Leucemia Mieloide Aguda
/
Regulação Leucêmica da Expressão Gênica
/
Proteínas de Fusão Oncogênica
/
Química
/
Dedos de Zinco
/
Subunidade alfa 2 de Fator de Ligação ao Core
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2003
Tipo de documento:
Artigo
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