Molecular identification of human/goat xenogeneic model / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 634-637, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-261373
ABSTRACT
<p><b>OBJECTIVE</b>To identify the human hematopoietic stem cells from the human/goat xenogeneic model with molecular techniques.</p><p><b>METHODS</b>DNA and total RNA were extracted from 11 transplanted goat peripheral blood cells. Human CD(34), GPA and SRY genes were amplified with PCR in these samples, and CD(34), GPA mRNA transcripts were detected using RT-PCR in 5 and 6 goat peripheral blood cells, respectively. Southern blot analysis was performed in 8 goat DNAs to detect the human specific alpha-satellite sequence. Meanwhile FISH was also performed to detect the human cells in goat blood with a probe of human Y chromosome.</p><p><b>RESULTS</b>Human CD(34) and GPA genes could be detected with PCR in all the 11 goats, and SRY gene did in 5 goats transplanted with hematopoietic stem cells derived from male human babies. Southern blot showed that human specific alpha-satellite sequence was present in 8 goats. By RT-PCR, human CD(34) mRNA was detected in 5 experimental goats, GPA mRNA was found in the other 6 experimental goats and FISH assay showed that some peripheral blood cells of the human/goat xenogeneic model were positive.</p><p><b>CONCLUSION</b>Existence of human cells in the recipient goats was identified by molecular detection, which was feasible for the examination of human/goat xenogeneic models.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transplante Heterólogo
/
Glicoforinas
/
Cabras
/
Células-Tronco Hematopoéticas
/
Southern Blotting
/
Reação em Cadeia da Polimerase
/
Hibridização in Situ Fluorescente
/
Quimeras de Transplante
/
Transplante de Células-Tronco Hematopoéticas
/
Antígenos CD34
Limite:
Animais
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2002
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS