Determination of ETO interaction domain within nuclear receptor co-repressor / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 621-623, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-261377
ABSTRACT
<p><b>OBJECTIVE</b>To determine the ETO-interaction domain of nuclear receptor co-repressor (N-CoR) for abolishing the biological function of AML1-ETO.</p><p><b>METHODS</b>Ten different regions of N-CoR (N-CoRYs) were generated by means of polymerase chain reaction (PCR), and cloned into yeast expression plasmid pGADGL to construct pGADGL/N-CoRYs. The yeast two-hybrid technique and X-gal staining were used to determine the binding between the 10 different regions of N-CoR and ETO.</p><p><b>RESULTS</b>It was shown that the co-existence of 988-1,126 and 1,551-1,803 amino acid residues of N-CoRY was the ETO-interaction domains required for the binding with ETO.</p><p><b>CONCLUSION</b>Two domains of N-CoR that interact with two zinc fingers of ETO, and keep stable binding between the two proteins were identified.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Ligação Proteica
/
Proteínas Repressoras
/
Fatores de Transcrição
/
Sítios de Ligação
/
Proteínas Recombinantes de Fusão
/
Proteínas Nucleares
/
Transfecção
/
Química
/
Proteínas Proto-Oncogênicas
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2002
Tipo de documento:
Artigo
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