Expression and clinical significance of anti-apoptosis gene, survivin, in acute leukemia / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 251-253, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-261438
ABSTRACT
<p><b>OBJECTIVE</b>To explore the correlation between expression of surviving gene in acute leukemic cells and its clinical effects.</p><p><b>METHODS</b>By using semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) technique, surviving gene expression in 50 previously untreated acute leukemia (AL) patients was analysed. The apoptosis of primary leukemia cells cultured in vitro was assayed with terminal deoxyribonucleotidyl transferase mediated dUTP-biotin nick end labeling (TUNEL).</p><p><b>RESULTS</b>Surviving gene expression levels in cells of AL patients at diagnosis were significantly higher than that in normal bone marrow mononuclear cells (MNCs) (82.0% vs 33.3%, P < 0.05). The expression level was higher in ALL cells than in ANLL cells (89.5% vs 75.0%). Among 22 cases of ANLL, bone marrow remission (BMR) rate was higher in surviving gene negative expression cells from patients accepted a course of chemotherapy than in positive expression cells (83.3% vs 25.0%, P = 0.023). Among 13 ANLL patients received a course of HA regimen chemotherapy, the BMR was higher in patients surviving mRNA negative expression cells than in positive cells (100.0% vs 27.3%). Patients with surviving/beta-actin ratio>0.6 attained lower BMR.</p><p><b>CONCLUSION</b>Higher expression level of surviving mRNA in AL cells may be one of the reasons that leukemic cells are insensitive to chemotherapy.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Indução de Remissão
/
RNA Mensageiro
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Proteínas Cromossômicas não Histona
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Leucemia Mieloide Aguda
/
Regulação Leucêmica da Expressão Gênica
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Resultado do Tratamento
/
Resistencia a Medicamentos Antineoplásicos
/
Usos Terapêuticos
/
Tratamento Farmacológico
Limite:
Adolescente
/
Adulto
/
Idoso
/
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2002
Tipo de documento:
Artigo
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