Study on gene expression changes of arsenic sulfide treated NB4 cells / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 16-18, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-261449
ABSTRACT
<p><b>OBJECTIVE</b>To compare the gene expression status of NB4 cells before and after arsenic sulfide treatment by cDNA microarray.</p><p><b>METHODS</b>Two cDNA probes were made from mRNA of untreated or arsenic sulfide treated NB4 cells. The cells were labelled with Cy3 or Cy5 fluorescence dyes individually, hybridized with cDNA microarray, and scanned for fluorescent intensity. The altered gene expression was screened through the analysis of difference in gene expression profile.</p><p><b>RESULTS</b>Thirty four genes related to apoptosis, cell cycle and others expressed different after the treatment of arsenic sulfide, 28/34 were up-regulated, 6/34 down-regulated.</p><p><b>CONCLUSION</b>ABC50, PNAS-2 and cyclin G(2) might take part in the process of NB4 cell apoptosis induced by arsenic sulfide.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Arsenicais
/
Sulfetos
/
Células Tumorais Cultivadas
/
Regulação Neoplásica da Expressão Gênica
/
Análise de Sequência com Séries de Oligonucleotídeos
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2002
Tipo de documento:
Artigo
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