Simultaneous HPLC determination of 11 essential compounds in Xuebijing injection / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2395-2398, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-262152
ABSTRACT
<p><b>OBJECTIVE</b>To establish a HPLC method for the determination of 11 essential compounds in Xuebijing injection.</p><p><b>METHOD</b>Chromatogaphic analysis was performed on Aglient Zorbax SB-C18 (4.6 mm x 250 mm, 5 microm). The mobile phase 0. 5% acetic acid and methanol-acetonitrile-acetic acid (40 60 0.5). The flow rate was 1.0 mL x min(-1) with the detection wavelength at 280 nm; column temperature at 35 degrees C, and automatic sampling volume of 20 microL.</p><p><b>RESULT</b>All the 11 essential compounds showed good linearity (r = 0.9982-0.9999)in the range of the tests concentration. The RSD of the precision, reproducibility and stability tests were less than 3%; the average recoveries of the method were in the range of 95.02%-104.94%.</p><p><b>CONCLUSION</b>The method is simple, rapid and accurate, so it is proposed for the quality control of compounds of Xuebijing injection.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Medicamentos de Ervas Chinesas
/
Cromatografia Líquida de Alta Pressão
/
Formas de Dosagem
/
Métodos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2010
Tipo de documento:
Artigo
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