Determination of chlorogenic acid and mangiferin in Folium Pyrrosiae from different habitats and species by HPLC / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 2075-2078, 2010.
Article
em Zh
| WPRIM
| ID: wpr-262222
Biblioteca responsável:
WPRO
ABSTRACT
An HPLC method for simultaneous determination of chlorogenic acid and mangiferin in original medicinal materials and decoction pieces of Pyrrosiae Folium was developed. The assay was performed on a Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column eluted with a mobile phase consisted of acetonitrile and 0.5% phosphoric acid solution in gradient elution at a flow rate of 1.0 mL x min(-1). The column temperature was set at 25 degrees C. The detection wavelength was 320 nm. The results showed that The linear ranges of chlorogenic acid and mangiferin were 5.2-130 mg x L(-1) (r = 0.9999) and 1.2-18 microg x mL(-1) (r = 0.9999), and the average recoveries (n=6) were 97.9% (RSD 1.9%) and 99.6% (RSD 2.9%), respectively. The method was simple, reproducible and valid. It can be used for quality evaluation and control of original medicinal materials and decoction pieces of Pyrrosiae Folium.
Texto completo:
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Índice:
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Assunto principal:
Plantas Medicinais
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Química
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Ácido Clorogênico
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Reprodutibilidade dos Testes
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Cromatografia Líquida de Alta Pressão
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Xantonas
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2010
Tipo de documento:
Article