Your browser doesn't support javascript.
loading
Abnormal mitochondrial function impairs calcium influx in diabetic mouse pancreatic beta cells / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 502-510, 2012.
Artigo em Inglês | WPRIM | ID: wpr-262582
ABSTRACT
<p><b>BACKGROUND</b>Abnormal insulin secretion of pancreatic beta cells is now regarded as the more primary defect than the insulin function in the etiology of type 2 diabetes. Previous studies found impaired mitochondrial function and impaired Ca(2+) influx in beta cells in diabetic patients and animal models, suggesting a role for these processes in proper insulin secretion. The aim of this study was to investigate the detailed relationship of mitochondrial function, Ca(2+) influx, and defective insulin secretion.</p><p><b>METHODS</b>We investigated mitochondrial function and morphology in pancreatic beta cell of diabetic KK-Ay mice and C57BL/6J mice. Two types of Ca(2+) channel activities, L-type and store-operated Ca(2+) (SOC), were evaluated using whole-cell patch-clamp recording. The glucose induced Ca(2+) influx was measured by a non-invasive micro-test technique (NMT).</p><p><b>RESULTS</b>Mitochondria in KK-Ay mice pancreatic beta cells were swollen with disordered cristae, and mitochondrial function decreased compared with C57BL/6J mice. Ca(2+) channel activity was increased and glucose induced Ca(2+) influx was impaired, but could be recovered by genipin.</p><p><b>CONCLUSION</b>Defective mitochondrial function in diabetic mice pancreatic beta cells is a key cause of abnormal insulin secretion by altering Ca(2+) influx, but not via Ca(2+) channel activity.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Cálcio / Secreções Corporais / Diabetes Mellitus / Eletrofisiologia / Células Secretoras de Insulina / Potencial da Membrana Mitocondrial / Insulina / Metabolismo / Camundongos Endogâmicos C57BL Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Inglês Revista: Chinese Medical Journal Ano de publicação: 2012 Tipo de documento: Artigo

Similares

MEDLINE

...
LILACS

LIS

Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Fisiologia / Cálcio / Secreções Corporais / Diabetes Mellitus / Eletrofisiologia / Células Secretoras de Insulina / Potencial da Membrana Mitocondrial / Insulina / Metabolismo / Camundongos Endogâmicos C57BL Tipo de estudo: Estudo prognóstico Limite: Animais Idioma: Inglês Revista: Chinese Medical Journal Ano de publicação: 2012 Tipo de documento: Artigo