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Endothelial progenitor cell down-regulation in a mouse model of Kawasaki disease / 中华医学杂志(英文版)
Chinese Medical Journal ; (24): 496-501, 2012.
Artigo em Inglês | WPRIM | ID: wpr-262583
ABSTRACT
<p><b>BACKGROUND</b>Cardiovascular complications of Kawasaki disease (KD) are a common cause of heart disease in pediatric populations. Previous studies have suggested a role for endothelial progenitor cells (EPCs) in coronary artery lesions associated with KD. However, long-term observations of EPCs during the natural progression of this disorder are lacking. Using an experimental model of KD, we aimed to determine whether the coronary artery lesions are associated with down-regulation of EPCs.</p><p><b>METHODS</b>To induce KD, C57BL/6 mice were administered an intraperitoneal injection of Lactobacillus casei cell wall extract (LCWE; phosphate buffered saline used as control vehicle). Study groups included group A (14 days following LCWE injection), group B (56 days following LCWE injection) and group C (controls). Numbers of circulating EPCs (positively staining for both CD34 and Flk-1 while staining negative for CD45) were evaluated using flow cytometry. Bone marrow mononuclear cells were cultured in vitro to expand EPCs for functional analysis. In vitro EPC proliferation, adhesion and migration were assessed.</p><p><b>RESULTS</b>The model was shown to exhibit similar coronary artery lesions to KD patients with coronary aneurysms. Numbers of circulating EPCs decreased significantly in the KD models (groups A and B) compared to controls ((0.017 ± 0.008)% vs. (0.028 ± 0.007)%, P < 0.05 and (0.016 ± 0.007)% vs. (0.028 ± 0.007)%, P < 0.05). Proliferative, adhesive and migratory properties of EPCs were markedly impaired in groups A and B.</p><p><b>CONCLUSION</b>Coronary artery lesions in KD occur as a consequence of impaired vascular injury repair, resulting from excess consumption of EPCs together with a functional impairment of bone marrow EPCs and their precursors.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Fisiologia / Células-Tronco / Adesão Celular / Movimento Celular / Células Cultivadas / Biologia Celular / Células Endoteliais / Proliferação de Células / Citometria de Fluxo Limite: Animais Idioma: Inglês Revista: Chinese Medical Journal Ano de publicação: 2012 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Fisiologia / Células-Tronco / Adesão Celular / Movimento Celular / Células Cultivadas / Biologia Celular / Células Endoteliais / Proliferação de Células / Citometria de Fluxo Limite: Animais Idioma: Inglês Revista: Chinese Medical Journal Ano de publicação: 2012 Tipo de documento: Artigo